Translational_Unit

Part:BBa_K322123:Design

Designed by: Chris French and Richard Edward Partridge-Hicks   Group: iGEM10_Edinburgh   (2010-09-25)

Phycobilin synthesis operon without terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is the same as BBa_K322122 except it doesn't have a terminator.

Source

This is derived from K098010 but PCR was used to remove the terminator, so that cph8 could be added at the end of the operon.


References